项目经历

电穿孔在伤口愈合中的应用

▪       通过高压微秒脉冲电场PEF对细胞膜的破坏促进细胞骨架的自组装

▪       增强细胞骨架的组装，降低细胞粘附性，从而能够增强细胞迁移

▪       同时脉冲电场能够诱导细胞增殖以及分泌促进伤口愈合相关蛋白

▪       PEF促进细胞骨架的自组装后增强其对细胞外基质的收缩性，促进伤口闭合

▪       PEF能够对细胞外基质中的胶原蛋白具有诱导重排的功能

可控纳米电穿孔在体内体外药物递送中的应用                                                                                                                                                                                                                                                                                                                                                         

▪       通过SEM及仿真结果可以看到在不同电参数下细胞表面产生不同大小和数目的细胞膜纳米级穿孔

▪       并且穿孔情况与可以电剂量进行对应，从而对细胞膜穿孔进行可控调制

▪       在体外细胞实验中，通过混合脉冲波形增强量子点、小分子、大分子的递送效率

▪       在体内动物实验中，同样发现混合脉冲对药物递送的促进作用

中频电场的电磁效应在脑肿瘤治疗中的应用

▪       通过有限元仿真不同频率的电场对细胞有丝分裂不同阶段的电场极化分布影响

▪       结果显示中频所产生的电磁效应显著增强，解释了目前肿瘤治疗电场（TTF）治疗过程选频依据

▪       中频电场能够在有丝分裂期间对微管产生聚集，有效抑制胶质母细胞的有丝分裂过程，降低细胞活性

▪       中频电场能够有效抑制胶质母细胞的迁移能力，并且抑制效果与电场强度成正比

掌握技能

▪ 细胞生物学：细胞培养、活细胞动态追踪、免疫荧光、蛋白定量（WB、ELISA）、成像技术（SHG、TIRF、Confocal）

▪ 电子信息学：电路设计、电路仿真模拟（PSpice）、有限元仿真（COMSOL对电穿孔及电场分布进行仿真）

▪ 微流控生物芯片：光刻（套刻），制作梯度电场电极芯片（PDMS）

▪ 动物医学：动物麻醉、腹腔/皮下注射、肿瘤模型动物

▪ 数据分析：Origin、SPSS、MATLAB

已获得的专利

▪ 刘克富，刘妍君，项骁炜，刘昊天，陶晓楠一种可形成梯度电场电刺激的细胞培养皿（实用新型），专利号：ZL202220038105.6

▪ 刘克富，项骁炜，邱剑，赵晖，朱才会，刘昊天，陶晓楠一种细胞电穿孔装置（实用新型），专利号：ZL 2022 2 3261487.8

获得奖项

▪ 第七期复旦-云锋大健康基金专项基金资助（《可控纳米级电穿孔介导的药物递送应用》，10万元）

▪ 2022年复旦大学“挑战杯”创新创业大赛一等奖

▪ 第八届中国国际“互联网+”大学生创新创业大赛上海市赛铜奖

▪ 2022年复旦大学博士生论坛二等奖及优秀墙报奖

▪ 复旦大学优秀毕业生

学术成果（* IndicatesEqual Contribution, ‡Corresponding Author）

▪   Xiao-Wei Xiang*, Hao-Tian Liu*, Ze-Yao Yan, Yu-Lian Zeng, Ya-Jun Wang,Wei Liu, Yu-Chen Chen, Yang Du, Sai-Xi Yu, Yi Wu, Xin-Xin Xu, Jiong Ma, Ke-FuLiu‡, Yan-Jun Liu‡. Revolutionizing Wound Healing: UltrashortPulse Electric Fields in Seconds for Highly Aligned Extracellular Matrix andEfficient Cell Migration. Chemical Engineering Journal 2023, 471: 144267.

▪   Xiang XW*, Liu HT*, Tao XN*, Zeng YL, Liu J, Wang C, Yu SX, Zhao H‡, Liu YJ‡, Liu KF‡. Glioblastoma behavior study underdifferent frequency electromagnetic field[J]. iScience, 2023, 26(12): 108575.

▪   Yixin Liu*, Zihui Fan*, Xiao-Wei Xiang*, Xiaonan Tao, XinweiXia, Qian Shi, Yanwei Lu, Jiayin Lu, Hongzhou Gu, Yan-Jun Liu‡, Baohong Liu‡. "Engineering of Multivalent Membrane‐Anchored DNA Frameworks for PreciseProfiling of Variable Membrane Permeability During ReversibleElectroporation."Small Methods (2023): 2301198.

▪   Xiaowei Xiang*, Zhengliang Guo, Gaoyun Fang, Yang Liu,and Jing Liu‡. TSAF: Two-StageAlignment Framework for Unsupervised Domain Adaptation. IEEE SIGNALPROCESSING LETTERS, 2023, 4, 698 – 702

▪   H. Liu*, X. Xiang*, X. Tao, H. Zhao, J. Qiu and K. Liu,"An Electroporation-mediated Intracellular Delivery Model with CoupledPore Currents and Solute Transport," in IEEE Transactions onDielectrics and Electrical Insulation, doi: 10.1109/TDEI.2024.3400770

▪   Xiao-Nan Tao*, Hao-Tian Liu*, Xiao-Wei Xiang‡, Cai-Hui Zhu,Jian Qiu, Hui Zhao, Ke-Fu Liu*, Regulating the Distribution and Accumulation ofCharged Molecules by Progressive Electroporation for Improved IntracellularDelivery. ACS Applied Materials & Interfaces, 2024, 16(28):36063-36076.Corresponding author

▪   Y Liu*, YJ Wang*, Y Du, W Liu, X Huang, Z Fan, J Lu, R Yi, XWXiang, X Xia, H Gu, YJ Liu‡.DNA nanomachines reveal an adaptive energy mode in confinement-induced amoeboidmigration powered by polarized mitochondrial distribution. Proceedings ofthe National Academy of Sciences, 2024, 121(14), e2317492121.

▪   Wei Liu*, Jia-Yin Lu, Ya-Jun Wang, Xin-xin Xu,Yu-Chen Chen, Sai-Xi Yu, Xiao-Wei Xiang, Xue-Zhu Chen, Yaming Jiu, HaiGao, Meng-Yao Sheng, Zheng-Jun Chen, Xinyao Hu, Dong Li, Paolo Maiuri, XinxinHuang, Tianlei Ying, Guo-Liang Xu, Dai-Wen Pang, Zhi-Ling Zhang, Baohong Liu,and Yan-Jun Liu‡. Vaccinia virusinduces EMT-like transformation and RhoA-mediated mesenchymal migration. Journalof Medical Virology 2023;95:e29041

▪   Yu-Lian Zeng*, Yang Du*, Xin-Xin Xu, Ya-Jun Wang, Sai-Xi Yu, Ting Liu, Shiyuan Luo,Xiao-Wei Xiang, Wei Liu,Yu-Chen Chen, Hongjun Huang, Hai Gao, Yuhui Shen, Yan Luo, Chengrong Bao, Yan-Jun Liu‡. On-chip modeling of physiological andpathological blood-brain barrier microenvironment for studying glial responsesto neuroinflammation. Nano Today, 2023, 52: 101947.

▪   Zhu, Caihui*, Li Yin, Jianzhi Xu, Xingjian Yang, HaoWang, Xiaowei Xiang, Haotian Liu, and Kefu Liu‡. 2022. Characteristics of Collagen Changesin Small Intestine Anastomoses Induced by High-Frequency Electric FieldWelding[J]. Biomolecules 2022, 12, 1683.

▪   C Zhu*, L Yin, J Xu, H Liu, X Xiang, HZhao, J Qiu, K Liu‡.An ex vivo preliminary investigation into the impact of parameters on tissuewelding strength in small intestine mucosa-mucosa end-to-end anastomosis[J]. Frontiersin Bioengineering and Biotechnology, 2023, 11: 1200239.

▪   H. Liu*, X. Tao*, X. Xiang*, H. Zhao, J. Qiu and K. Liu‡, "Modelling of Cell Electroporationwith Emphasis on Ions Transport-induced Dynamic Alterations in MembraneConductivity," in IEEE Transactions on Dielectrics and ElectricalInsulation, doi: 10.1109/TDEI.2023.3327218.

▪   Wang Yifan*, Liu Kefu‡, Xiang Xiaowei, Zhu Caihui, WangHao, Yin Li, Yang Xingjian. The impedance analysis of small intestine fusion bypulse source[J].Open Life Sciences, 2020, 15(1): 808-818.

▪   Han, Z., Peng, C., Yi, J., Zhang, D.,Xiang, X., Peng, X., ... & Qiao, L. Highly efficient exosomepurification from human plasma by tangential flow filtration based microfluidicchip[J]. Sensors and Actuators B: Chemical, 2021, 333: 129563.

▪   Wang, T*‡., Cao, Z., Shen, Z., Yang, J., Chen, X.,Yang, Z., Xu, K., Xiang, X., Yu, Q., Song, Y., Wang, W., Tian, Y., Sun,L., Zhang, L., Guo, S., & Zhou, N. (2020). Existence and functions of akisspeptin neuropeptide signaling system in a non-chordate deuterostomespecies[J]. eLife, 9, e53370.

▪   Xiaowei Xiang*, Muyan Chen, Changwen Wu, Jingwen Yang,Zhenming Lv, Tianming Wang‡.Glycolytic regulation in aestivation of the sea cucumber Apostichopusjaponicus: Evidence from metabolite quantification and rate-limiting enzymeanalyses[J]. MARINE BIOLOGY, 2016, 163(8): 1-12.

Underreview

▪   Xiao-Wei Xiang*, Xiao-Nan Tao*, Hao-Tian Liu, Cai-Hui Zhu, Jing Liu,Ya-Jun Wang, Wei Liu, Yu-Chen Chen, Yu-Lian Zeng, Sai-Xi Yu, Jian Qiu, GuangyinJing, Hui Zhao, Qi-Hong Huang, Yan-Jun Liu, Ke-Fu Liu, ProgressiveElectroporation Enhances Intracellular Delivery Efficiency. ACS Nano, 2024 (under review).